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the problem is how can to analyse Gentamicin by using
HPLC instrument in spite of our knowing that
gentamicin does not absorp in UV region, If you have
any method that use HPLC please send us.
with best regards and more development
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To Naim Habil,
There are several gentamicin HPLC methods available. You did not
describe what you need to analyze (serum, urine, tissue etc.). We
recently published a serum and urine method:
Isoherranen & Soback: Determination of gentamicins C1, C1a and C2 in
plasma and urine by HPLC. Clinical Chemistry, 2000, 46,837-842.
You have to take to account that every method will use derivatization
(unless you use LC/MS). It may be helpful to know the chemistry
behind the derivatization to avoid confusion in the results. However,
this should not scare you off because in our experience at least our
method is fairly easy to perform and provides reproducible results.
The major problem, however, is that gentamicin is not a single
compound but a combination of three major components. Finding
reference material of the components is practically impossible and
apparently you have to isolate the compounds by yourself. The
concentration of all commercial reference material (mixture of the
components) is based on antimicrobial activity and has, therefore, no
real correlation to the concentration of the chemicals in the complex.
Let me take this opportunity to convey my deepest sympathy to our
American colleagues and nation.
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