Back to the Top
dear all
i want to administer a solution formulation through intratracheal route
in a rat. could someone suggest the voulme of solution formulation
allowed with out inflicting significant physiological alteration in the
lung. moreover what about drugs whose LogP is very high and have poor
solubility in water or saline. can we add any solubility enhancers like
PEG, or any other surfactant like sodium dodecyl sulfate/laural sulfate.
if so would the use of these surfactants have any affect on the
pulmonary environment. also please suggest the conc of the allowed
surfactant. somebody kindly suggest something in this regard. any
suggestions are welcome and would be really appreciated.
thanking u
manish
department of pharmaceutics
univ of florida
Back to the Top
The following message was posted to: PharmPK
We have used 200 =B5L via intratracheal instillation. This seems to be about
the maximum volume you can administer safely. Yes you can administer
surfactants and other solubilizing agents as well as up to 85% ethanol in
water. Pulmonary phospholipid based surfactants are best. Avoid the Tweens,
Spans and Cremaphor. With intratracheal administration and the volumes
suggested you will not involve more than the 2nd/3rd bifurcation of the
bronchial tree and consequently most of the lung is not involved. As far as
impact to the bronchial lining etc is concerned we have not noticed any
changes even under histopathology. As far as concentrations of the
surfactants we have used up to 15% PEG in water. Good Luck with your
research.
Anup Zutshi Ph.D.
Pharmacokinetics and Metabolism
Pharmaceutical Product Development
Battelle Memorial Institute
Tel.No: (614) 424-5997
=46ax: (614) 424-3268
E-Mail: zutshi.-at-.battelle.org
Back to the Top
The following message was posted to: PharmPK
hi manish!
I have faint idea that your formulation could be a
suspention and you could calculate the dose on the
basis of body weight. I have seen some journals on
intratracheal administration but the sampling is thro'
microdialysis. i dont know if thats the procedure you
would like to follow. let me know..
deepak bhatia
st john's university.
Back to the Top
Dear All
i would like to clarify another problem of mine. we were trying to
administer DPI using a Dry powder insufflator device from pen century
for non-invasive administration of dose. the problem we r facing is
that we are not able to place the Stainless Steel probe tubing into
the trachea inspite of all our efforts. it appears that the dose goes
into the GIT. Is there an easy way to confirm that the intratracheal
tube would be inside the trachea and not in the GIT. when we
administered the dose after opening the trachea the results we got
were positive. it showed that the dose was not going into the lung.
please suggest some tips in this regard.
thanks
manish issar
Back to the Top
The following message was posted to: PharmPK
You can verify that the tube is in the trachea by angling the end of the SS
tube up and feeling for it with your finger on the rats neck. If you don't
feel the end clearly you should pull it back and try again.
Good Luck.
James D. Talton, Ph.D.
President & CEO
Nanosphere, Inc.
12085 Research Drive, Suite N
Alachua, FL 32615
(904) 462-9663
(904) 462-0875 fax
www.nanosphere.com
Back to the Top
The following message was posted to: PharmPK
Dear Manish,
You should put an intratracheal probe near the
tracheal bifurcation or use a "laryngoscope" when you
are dosing the animal (who must be in vertical
position for approximatelty one minute in order to
avoid reflux or regurgitation).
PharmPK Discussion List Archive Index page
Copyright 1995-2010 David W. A. Bourne (david@boomer.org)