PharmPK Discussion - Ketoprophen

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On 17 Sep 2001 at 13:45:43, "Ivan Kovacevic" (ivan.at.pharmacy.bg.ac.yu) sent the message


The following message was posted to: PharmPK

Dear group,

I would appreciate any information connected with extraction procedure and
HPLC
analysis/UV detection of ketoprophen in plasma (blood). I am not interested
in
measuring S and R enantiomers. I need these information in order to conduct
ketoprophen bioavailability assay.

Thank you in advance for any information you will send to me.
Ivan Kovacevic
Teaching/research assistant,
Department of Pharmacokinetics,
Faculty of Pharmacy, Yugoslavia
e-mail:          ivan.-at-.pharmacy.bg.ac.yu

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On 25 Sep 2001 at 23:01:07, kwon.at.cnu.ac.kr sent the message


Ivan
Concerning the Ketoprofen HPLC analysis and extraction, we could
measure ketoprofen with sensitivity of 1ng/ml in plasma after
applying ketoprofen cream or pasta(90mg/man) on arm skin in human
volunteer. Extraction : plasma 0.5ml, internal standard flubiprofen
500ng/ml in methanol 0.5ml - extract this 1 ml with 10 ml ether for
10 minutes and centrifuse and dry upper layer and dissolve with 250ul
of mobile phase.  Mobile phase : 0.02M NaH2PO4 buffer pH 3.0  :
acetonitrile (60:40).  HPLC : Schimazu 10A HPLC system, UV detector
256nm, Culumn : reverse phase C18(60A, 4um, 3.9x150mm), flow rate
1ml/min. Results : Retention time, ketoprofen 4.7min, flubiprofen 9.8
min, detection limit 1 ng/ml, extraction 98%. Reference in Korean :
Yakhak Hoeji Vol. 42. No.1 Page 25-30(1998).
Hope this would be help

Kwon K.I.
Professor in College of Pharmacy, CNU, Korea
kwon.-a-.cnu.ac.kr

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