PharmPK Discussion - Perfused bovine eye

• On 6 Aug 2001 at 23:32:28, Koeberle_Martin (Koeberle_Martin.at.Allergan.com) sent the message
  

  
  The following message was posted to: PharmPK
  
  Dear all,
  
  I plan to use an arterially perfused bovine eye preparation to study the
  kinetics of an opthalmic drug. In the experiment I perfuse the eye at a
  constant flow, but the matter is to decide on the optimum flow-rate. I tend
  to use a flow rate of about 1ml/min or slightly above, however, I haven't
  found any literature about the physiological blood flow in bovine eyes. In
  literature about arterially perfused bovine eyes flow rates between 0.8 and
  2.4 ml/min were used, but none had a reference to what's physiological. Has
  anyone an idea about it or done something similar?
  
  Thanks,
  
  Martin Koeberle
  Department of Pharmaceutical Sciences
  University of Strathclyde
  Glasgow, UK
  
  

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• On 7 Aug 2001 at 11:23:12, "olaf.kuhlmann" (olaf.kuhlmann.at.medizin.uni-halle.de) sent the message
  

  
  The following message was posted to: PharmPK
  
  Dear Martin,
  
  I don't have any information about the bovine perfusion rate, but there is a
  paper about the arterial flow in the human:
  BEUNINGEN EG VAN, FISCHER FW, 1957
  Clinical determination of pulse volumen and basic arterial flow of the eye.
  Klin. Mbl. Augenheilkunde 131(1)57-61
  The article is in German, but the main point is that per pulse 17-22 =B5L we=
  re
  transported into the eye. So if you know the pulse (e.g. 70/min) you can
  calculate the flow (1.2-1.5 mL/min).
  
  Hope this helps,
  
  OLAF
  
  olaf.kuhlmann.aaa.medizin.uni-halle.de
  
  

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