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The following message was posted to: PharmPK
Have you labeled the variable portion to see where its going?
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The following message was posted to: PharmPK
Dear Gordon,
if you do not find the antibody fragment in urine, it could also be
taken up in the kidney. Then it is likely to be metabolised in the
lysosomes of the kidney. If you have not for examples bound a radiolabel
to it, you might not detect any remnant from the protein except
aminoacids. There are several comprehensive reviews on this topic
available, e.g in Clinical Pharmacokinetics, by Blumenthal etc.
Kind regards,
Dietrich
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Dear Edward
We dont have a radiolabel for the recombinant antibody (yet). Our sole
detection method is by detecting a 6xHis tag at the carboxy terminal. I
guess its possible that the tag is being cleaved/processed. Any thoughts
?
Thanks and all the best
Gordon
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Hi,
An article in the Quarterly Journal of Nuclear Medicine 1998 42:225-41
by Colcher et al may be a good source.
My guess is that there may be a high renal uptake, metabolic degradation
followed by tubular re-absorption and/or secretion. However, depending
on the size, the catabolism of this antibody could take place in the
liver (since the catabolism of larger proteins takes place primarily in
the liver).
Best Regards,
Ying Ou
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