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How I can make the analysis of risperidone from samples of urine?. I
have HPLC instrumentation. It is necessary to make some extraction
Tkank you very much
Dr. Marco A. Quiroz
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Here is one assay I found after doing a quick search. I realize it was for
serum, but you should be able to adapt it for urine.
Louis R. Ptak, Ph.D.
Senior Medical Writer
ICON Clinical Research
RISPERIDONE ASSAY BY HPLC-UV DETECTION
S. Athwal, P.J. Streete
Medical Toxicology Unit, Guy's & St Thomas' Hospital Trust, Avonley Road,
London. SE14 5ER, UK
Risperidone is a new antipsychotic agent first introduced in 1990.
Risperidone acts through selective antagonism of serotonin 5HT2 and dopamine
D2 receptors. It undergoes hydroxylation to yield 9-hydroxyrisperidone, an
active metabolite that has similar pharmacological activity to that of the
parent drug. Hydroxylation is under the control of the polymorphic CYP2D6
enzyme. Factors that are known to influence serum concentrations of the
active moiety include age, impaired renal function, CYP2D6 genotype and
co-medication. Although there is no clear correlation between serum
concentrations and clinical effects, there are certain situations where
monitoring of risperidone levels may be of value. These include the
evaluation of non-compliance and the investigation of adverse drug
reactions, for example high doses of risperidone can induce extrapyramidal
At the MTL, we have adapted a simple and sensitive HPLC method with UV
detection for the quantification of risperidone and 9-hydroxyrisperidone.
After sample alkalinization the test compounds are extracted from serum
using methyltertiarybutylether:isoamylalcohol (99:1 v/v). The organic phase
is back extracted into an acidic phosphate buffer, an aliquot of which is
injected onto a Hypersil ODS analytical column (5Ám, 250 x 4.6 mm I.D.). The
compounds are detected at 278 nm. The mobile phase consists of phosphate
buffer (0.05M, pH 3.7):acetonitrile (55:45 v/v), pumped at a flow rate of
1.5 mL/min. Data derived from the therapeutic monitoring service will be
Keywords: Risperidone, 9-Hydroxyrisperidone, HPLC, therapeutic monitoring.
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