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The following message was posted to: PharmPK
I WOULD APPRECIATE IT IF YOU COULD PROVIDE ME WITH A SOURCE OF
INFORMATION
ON THE PHYSICOCHEMICAL PROPERTIES OF GLUCOSAMINE HYDROCHLORIDE AND
SULPHATE
AND CHONDROITIN SULPHATE AND ASSAY METHODS FOR THESE COMPOUNDS.
REGARDS
NAFISAH BEGUM
R & D CHEMIST
BEACONS PHARMACEUTICALS
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Hello Nafisha,
A validated UV-HPLC method using pre-column derivatization with
phenylisocyanate has been reported for determination of Glucosamine HCl
in plasma, kindly refer
Liang Z, Leslie J, Adebowale A, Ashraf M, Eddington ND. Determination
of the nutraceutical,glucosamine hydrochloride, in raw materials,
dosage forms and plasma using pre-column derivatization with
ultraviolet HPLC.
J Pharm Biomed Anal. 1999 Sep;20(5):807-14.
Assay of Chondroitin sulfate has beenperformed that detects
disaccharides formed from Chondroitin sulfateafter treatment with
Chondroitinase ABC, refer
Volpi N. Disaccharide analysis and molecular mass determination to
microgram level of single sulfated glycosaminoglycan species in
mixtures following agarose-gel electrophoresis. Anal Biochem. 1999 Sep
10;273(2):229-39.
and
Adebowale A, Du J, Liang Z, Leslie JL, Eddington ND.
The bioavailability and pharmacokinetics of glucosamine hydrochloride
and low molecular weight chondroitin sulfate after single and multiple
doses to beagle dogs.
Biopharm Drug Dispos. 2002 Sep;23(6):217-25.
Hope this info. will help you,
good luck
VijayV. Upreti.
Graduate Student , Department of Pharmaceutical Sciences
School of Pharmacy, University of Maryland. 20 Penn St., Baltimore. MD
21201
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