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The following message was posted to: PharmPK
Does anyone know of a commercially available system for performing cell
metabolism, uptake and survival (clonogenicity) experiments in stirred
cell suspensions with O2/N2/CO2 gassing to control pH? Or,
alternatively, the designs of good custom system you are willing to
We are looking at upgrading our system. One of the major problems with
the current system is setting a consistent and reliable gas flow.
Ideally we require:-
*Magnetic or other stirring system
*Vials with gas inflow and outflow ports and sampling port.
*Accurate and reliable gas flow setting that minimizes evaporative loss,
temperature and pressure fluctuations
*Efficient gassing system allowing accurate control of solution oxygen
*Possibly with inline gas meters and/or an alarm if gassing of a vial
falls below a set rate.
*Ability to monitor pH, pO2 etc by insertion of electrodes into sampling
*Easily cleaned and sterilised
If anyone knows of any good systems in use I would be very grateful to
hear from them.
Kevin Hicks B.V.Sc., Ph.D.
Experimental Oncology Group,
Auckland Cancer Society Research Centre,
The University of Auckland,
Private Bag 92019,
Ph: 64 9 373-7599 Ext: 86939
Fax: 64 9 373-7571
Home: 64 9 629 0364
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