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I am performing radioligand receptor binding assays of NCEs on some
I dissolve compounds in 100% DMSO and then Further serially dilute it
to 2% in water (final dilution). First we dilute to 50% DMSO then 10%
and so on.
This is because the final concentration of DMSO is not supposed to be
above 2% when in contact with receptors.
However the compounds I am dealing with are Highly insoluble in water.
So whenever we try to dilute it to 50% DMSO the compounds precipitate
or flake or become turbid.If this stage is pased and the compounds
remain solubilised then the dilutions dont cause an problem.
Can any body suggest me a solution to this problem? Has any body used
other solvents for radioligand binding assays?
All suggestions are welcomed is this regards
thanks in advance
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