Back to the Top
I am performing radioligand receptor binding assays of NCEs on some
I dissolve compounds in 100% DMSO and then Further serially dilute it
to 2% in water (final dilution). First we dilute to 50% DMSO then 10%
and so on.
This is because the final concentration of DMSO is not supposed to be
above 2% when in contact with receptors.
However the compounds I am dealing with are Highly insoluble in water.
So whenever we try to dilute it to 50% DMSO the compounds precipitate
or flake or become turbid.If this stage is pased and the compounds
remain solubilised then the dilutions dont cause an problem.
Can any body suggest me a solution to this problem? Has any body used
other solvents for radioligand binding assays?
All suggestions are welcomed is this regards
thanks in advance
PharmPK Discussion List Archive Index page
Copyright 1995-2010 David W. A. Bourne (firstname.lastname@example.org)