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The following message was posted to: PharmPK
hi all!
does anybody know on what factors one should decide which of the two
biological fluids, serum or plasma, should be chosen to carry out
pharmacokinetic studies?
thanks
shabana
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[Two replies - db]
From: "Dr. Thomas G. Baumgartner"
Date: Wed Feb 19, 2003 12:45:04 PM US/Central
To: david.aaa.boomer.org
Subject: PharmPK Serum or Plasma?
Reply-To: "Dr. Thomas G. Baumgartner"
Attachments: There is 1 attachment
Hi Shabana,
If the pharmacokinetic study isn't complicated by clotting factors then
plasma can be used.
If there is potential for interference with clotting factors - human
blood is left to coagulate; the clot is then removed before
centrifugation to provide serum (now without clotting factors) post
centrifugation.
Sincerely,
"TB"
---
From: bvatul.-a-.ufl.edu
Date: Wed Feb 19, 2003 7:20:18 AM US/Central
To: david.at.boomer.org
Subject: Re: PharmPK Serum or Plasma?
Reply-To: bvatul.at.ufl.edu
The following message was posted to: PharmPK
Hello Shabana
One important factor would be the presence of fibrin in plasma and you
might
want to check if your drug binds to it by comparing the recovery from
both
matrices.
Venkatesh Atul Bhattaram
Post-Doctoral Fellow
University of Florida
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The following message was posted to: PharmPK
Depending on how much of the drug is being sequestered by RBC. If it is
sequestered extensively, as in paclitaxel, then you need to look at
both.
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The following message was posted to: PharmPK
Shabana-
When considering to use either whole blood vs. serum/plasma, be sure to
evaluate if temperature-dependent binding to RBC's or plasma proteins
exists for the drug. Otherwise, you will introduce variability
depending on the temperature of separation (and centrifugation) and
comparisons between research groups may be difficult (since different
groups may separate blood cells at different temperatures.
Lane
Lane J. Brunner, Ph.D.
College of Pharmacy
The University of Texas at Austin
University Station, A1920 (USPS)
2409 West University Street (courier)
Austin, TX 78712-0126
Tel: (512) 471-0942
Fax: (512) 471-7474
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