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The following message was posted to: PharmPK
We are trying to determinate (by HPLC) TPMT activity in erythrocyte
lysate.
In according with different papers, erythrocyte lysate has to be
chelated with Chelex
100.
I want to know how to prepare it (Chelex 100) and the concentration to
be used in
incubation.
Thank you very much.
Guillermo Bramuglia
Paulo A. Caceres Guido
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Copyright 1995-2010 David W. A. Bourne (david@boomer.org)