I would like to know the answer about if the Glimepiride concentrationBack to the Top
in human serum, could we use HPLC with UV detector?
Dario Rojas B
Back to the Top
Yes. Definitely you can use HPLC with uv detector to estimate
glimepride in human plasma and/or serum. The lambda max is 230.
With warm regards
Wellquest Clinical Research
6th Wellspring Hospital
Mumbai 400 013
Tel(O) 91 22 5660 8566/8567 Ext 233
Dear Rojas,Back to the Top
U can definitely try to estimate the Glimepiride in Plasma by HPLC-UV
using derivatization process. I hope the same can be applied to serum
The best solution of determination of glimepiride in humanBack to the Top
plasma(serum) is LC/MS resp. LC/MS/MS method with liquid-liguid
extraction from acid environment. To use UV detection you must
derivatize the sample.
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