Dear groupBack to the Top
I am working on bioanalytical and estimation of newer analgesis in plasma.
in case of STD plot i am getting the IS area same in All concentration but
the area of drug is not proportional. it is totallly
different. extraction of the drug by precicpitation method .
If any one suggest me the answer.
Hi,Back to the Top
It is difficult to say the exact problem without knowing complete
information. But it seems that your drug is not constantly
extracted from plasma proteins at some concentrations unlike your
internal standard. So you can try different precipitation agents
like acetonitrile, methanol, 1% zinc sulfate, 0.05% TCA etc with
and without internal standard (to check the interference of IS).
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