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I'm a first year PhD student culturing rat hepatocytes
(primary culture) using modified L15 (Leibovitz) media
supplied by Sigma in 5%CO2. I'm culturing as spheroids, so
proliferation/long term storage is not required. I'm doing
toxin testing using both traditional biochemical (enzyme)
tests, and proteomic/genomic analysis.
Q1 - L15 is not meant to be used in CO2 - does anyone know
if there are any detrimental effects from using L15 in CO2?
Q2 - Sigma have stopped manufacturing media - I'm looking
for input for a suitable alternative (DMEM already
discovered to be not as good).
Q3 - I need to use protease inhibitors to protect proteins
for proteomic analysis. How will these affect my enzyme
assays (I'm measuring LDH, GPT, GOT, GGT).
Any input would be appreciated!
"University of the West of England"
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