Dear all,Back to the Top
I am trying to set up a system based in fluorometric quantitation for
CYP phenotyping. I chose AHMC \0x2021 AMMC reaction (AHMC :
for CYP2D6 activity evaluation and 390/460nm as wavelengths for
excitation/emission. I have observed that the substrate emits
fluorescence too when measuring at that wavelengths (approximately 1/6
of the product\0xEDs intensity).
Since our equipment cannot measure the excitation and emission spectra
of the compounds, could anybody tell me about them, or just if I should
measure at another wavelengths?
In case this is not possible, do you know ifthere is a better
substrate for the fluorometric evaluation of CYP2D6 activity (MAMC,
Thank you very much in advance.
You can also try Bufuralol. Ex/Em for the metabolite are = 252/302Back to the Top
Department of Pharmacology
Division of Experimental Therapeutics
Walter Reed Army Institute of Research
503 Robert Grant Ave. Room 1E22
Silver Spring, MD, 20910
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