PharmPK Discussion - Docetaxel metabolism

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On 12 Feb 2004 at 16:15:02, Elice Brooks (Elice.Brooks.-at-.uvm.edu) sent the message


Hello All,
I have a perplexing problem that I hope you can help me with.
We are treating Fisher 344 rats with docetaxel for pharmacokinetic
studies.
  I can extract the docetaxel from purchased blank rat plasma and pooled
blank plasma from our rats with an extraction efficiency of <80% by SPE
but
I can not detect docetaxel from any of the pharmacokinetic samples from
our
rats.  Our earliest time point is 1 minute.  Any thoughts?
Thank you.
Elice Brooks
Universtiy of Vermont
Vermont Cancer Center
32 North Prospect Street
Burlington, VT 05401
office (802)656-8352
lab    (802)656-8380
fax    (802)656-8333

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On 12 Feb 2004 at 14:09:00, "Vuong Trieu" (vtrieu.at.AmericanBioScience.com) sent the message
```
First, how was docetaxel administered?  Tween?
```
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On 13 Feb 2004 at 10:18:58, "Elena Strocchi" (strocchi.-a-.ms.fci.unibo.it) sent the message


Dear Elice,
first of all some questions:
- which docetaxel dose did you administer to rats and in which way (long
infusion or...) ?
- what's the limit of quantitation of your analytical method?
Bye
Elena
Elena Strocchi
Laboratorio di Farmacocinetica ANTLAB
Dipartimento di Chimica Organica
Universita di Bologna
Viale Risorgimento 4
40136 BOLOGNA  Italy
Tel. +39 0512093645  3483102781
FAX  +39 0512093654
email strocchi.aaa.ms.fci.unibo.it
WEB: www.antlab.org

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On 13 Feb 2004 at 12:05:05, (Iolanda.Cirillo.-at-.aventis.com) sent the message


Hi,
The pharmacokinetics of docetaxel have been studied extensively
preclinically, including rats. Administration was via intraperitoneal or
intravenous, using HPLC with UV and on-line radioactivity detection for
analysis.
I see that some have already asked you how docetaxel was administered
in your
study and the lower limit of quantitation of your assay; important
questions
to proceed.
Thanks

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On 13 Feb 2004 at 14:52:46, Elice Brooks (Elice.Brooks.aaa.uvm.edu) sent the message


Elena
The docetaxel was administered by iv bolus in the tail vein and the
limit
of quantitation for my assay is 0.1uM.
Elice
Elice Brooks
Universtiy of Vermont
Vermont Cancer Center
32 North Prospect Street
Burlington, VT 05401
office (802)656-8352
lab    (802)656-8380
fax    (802)656-8333

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