Dear Group,Back to the Top
We are working in the bioanalysis of salt form of a drug. How to
estimate the free drug present in plasma or seum as the drug will be
available as free form?
2.Can anyone give some suggestion about the extraction of aceclofenac
by liquid -liquid extraction in plasma by using UV detection?
I will be thankful for your answers.
Dear Rajesh,Back to the Top
It is always better to estimate the the free drug in plasma using the
drug rather than it's salt form if you are talking about in vitro
binding. I think there would be a difference in the extent of protein
binding of the base drug and its salt form in vitro. However this
might not be evident in vivo. I would be happy to hear from the group
Dear jagannathBack to the Top
What could be the reason for this difference in protein binding. I
appreciate if you suggest any references in this regard.
Dear Jayasagar,Back to the Top
The reason could be the in vitro ionisation of the salt form of the drug
thus reducing the chances of protein binding. I was also inclined to
an experiment with a compound and it's corresponding salt to get some
I am not in possession of the article which supports my assumption but i
think i had seen the article. I will be happy to hear from someone in
group to support or correct my assumption. I am keen to know about it
the pharmpk group. Thanks for your interest,
PharmPK Discussion List Archive Index page
Copyright 1995-2010 David W. A. Bourne (firstname.lastname@example.org)