Dear Group,Back to the Top
In doing drug transport study using rat gut sac technique, how can we
be sure of gut viability and integrity as well?
Dear Pravin,Back to the Top
The gut viability is an important assessment parameter in in situ
absorption studies. Cell viability of a gut can be checked by many staining techniques like tryptan blue dye exclusion method. Refereces are available in many literatures. One of the reference which mentions use of same is,
Koyo Nishida et al, Hepatic Disposition characteritstic of electrically
charged macromolecules in rat in vivo and in the perfused liver, Pharm
Res, Vol 8, No.4, pp-437-444, 1991
Hope this will help
Have a nice day
Kole Prashant, M.Pharm (Ph.D)
Department of Pharmacokinetics and Drug Metabolism
Birla Institute of Technology and Science, Pilani
Phone: (O) 01596-245074-Ext-458
Hi Parvin,Back to the Top
You can indirectly assess the viability by estimating the permeability
of glucose or any other actively transported markers.
Dear Parvin,Back to the Top
I have used the gut sac technique for many years as I learnt it in Dr
Woodley's laboratory, University of Keele. We always used glucose as a
marker for active transport and thus tissue viability and integrity.
However, some authors have questioned glucose transport ratio as a
measure of viability arguing that it is greatly influenced by
the tissue and thus gives variable results (Ruth Duncan et al) and they
gone on and used LDH as a marker.
I hope this helps.
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