Dear all,Back to the Top
In doing Everted gut sac study for drug transport, how can we measure
exact volume of solution inside the sac? Is it possible to use the
density of solution and the weight of the sac with and without
solution? By the way is the volume inside the sac constant during the
experiment? If not, need to use any marker (such as phenol red) for
this purpose? Any command will be appreciated.
Thanks in advance
Dear ParvinBack to the Top
I dont have any idea about the density method but what i think is that the
volume of solution inside the sac can be determined by measuring the
dimensions (length n radius) of the sac with a scale/thread and then
applying the formula for volume of cylinder. The volume inside the sac does
change during experiment and suitable marker is desired.
I hope this would help.
Research Associate, MAP-Discovery
Dear Parvin,Back to the Top
The most accurate way to measure a gut sac volume that I found is the
1. Weigh the sac before cutting it open
2. Cut it open to retrieve the media inside
3. Blot the sac dry on tissue paper (you will correct for volume lost in
4. RE-weigh the sac
I do not know which media you use (Tissue culture, PBS etc). By knowing the
weight and the density of your media, you can calculate accurately the
volume you had inside through the formula density =mass/volume.
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