PharmPK Discussion - In Situ Gut Perfusion

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On 17 Apr 2004 at 09:14:51, "Parvin Zakeri" (pzakeri.aaa.tbzmed.ac.ir) sent the message

Hi All,
I am working on drug permeability by in situ rat gut perfusion
technique. Can I work more than one drug in each experiment? That way
It will be done more faster and need fewer rats. Thanks in advance for
your reply.
Parvin

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On 18 Apr 2004 at 17:03:55, Kasiram Katneni (kasiram.katneni.-a-.vcp.monash.edu.au) sent the message

Hi Parvin,
You can estimate in-situ permeability of mixture of drugs (more than
one),
provided you know the absorption pathway of all the drugs in question
and is
by passive diffusion. If any drug is transported actively or is a
substrate
for efflux transporters, you cannot combine drugs as that will lead to
saturation of the active processes and is one form of drug-interaction.
Also, even though all the drugs in question are absorbed passively, if
huge
differences in concentration of individual drugs exist, that may
sometimes
lead to erroneous permeability estimates.
Kasiram.

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On 24 Apr 2004 at 10:10:26, "Parvin Zakeri" (pzakeri.aaa.tbzmed.ac.ir) sent the message

Dear group
Thank you for all your kind messages. Actually I am doing single pass
intestinal permeability experiments. I need to collect samples about
90-120 min after Canulation.

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On 6 May 2004 at 16:02:07, Krishna Kumar Machavaram (Krishna.Machavaram.-a-.vcp.monash.edu.au) sent the message

Hai Parvin,
I think you cannot do that because permeability of one drug may
influence
the other, that way you cannot get the actual transport patterns of the
drugs. There may be also possiblities for drug-drug (PK/PD )
interactions.
Thanks,
Krishna

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