Hi everyone,Back to the Top
I'm planning to undergo enzyme (CYP) induction studies in rats. Two
could be possible:
- Administration of the test substance to the animals for several days,
collection of the livers, preparation of microsomes and incubation with
-Exposure of hepatocytes ( commercially available) to the test
substance for 2-3 days and addition of CYP substrates to the media.
I would appreciate if someone could tell me which method would be
better for such studies.
Hai SamiaBack to the Top
If you are planning to do CYP induction study, It will better to do by
in vitro exposure of drug to hepatocytes first - For protocol refer to
In vitro techbologies.
If you find any thing interesting (positive induction) then you can
supplement with the in vivo data. All the best.
Ansar Ali Khan, M.Sc.,
Drug Metabolism and Pharmacokinetics,
Glenmark Research Centre,
Plot No.-A-607, TTC, Industrial Area,
Mahape, Navi Mumbai-400 709,
Phone No: 91-22-5590 2491/92 Extn.308
Fax No.: 91-22-5590 2318
SamiaBack to the Top
Start with defining what the question is that you are trying to address
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