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The following message was posted to: PharmPK
I have a question regarding Km/Vmax determinations. I have a compound
that is metabolized into two products. I am using the product
formation approach to determine Km/ Vmax. How is the Km/Vmax of one
metabolite differenciated from the other? Do I need to block the
formation of one metabolite in order to obtain the "true" Km/Vmax
value of the other? Is it possible to obtain a correct Km/Vmax value
by simply selecting an incubation timepoint (at initial rate
conditions) in which one metabolite is present while the other is not
present yet? How will the Km/Vmax of the other metabolite will be
calculated if the last scenario is used?
Thanks for your help!
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