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Before I embark on some mouse pharmacokinetic studeis for the first
time, i was wondering if i could ask a couple of questions.
What are the most commonly used strains of mice for these studies? What
are the reasons to choose one strain over another?
What is the concentration of heparin that should be used to heparinze
syringes prior to blood withdrawal?
Thanks so much for your help
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I am involved in PK mouse studies.
We use C57BL/6 mice from Jackson. They are easily obtained inbred
strain of mice that are commonly used.
If you are sacrificing your mice at the end of each time point then I
would suggest you used Terumo syringes w/o heparin to cardiac puncture
the mice. For use of an anticoagulant I commonly use BD microtainer
brand tubes w/K2EDTA. The BD # is 365974. These work great for our
analytical group. Heparin was interfering in the analysis of the
Hope this helps
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"What are the most commonly used strains of mice for these studies? WhatBack to the Top
are the reasons to choose one strain over another?"
We typically will use whatever strain of mice used in previous work.
Commonly used strains are C57, CD-1, CF-1.
"What is the concentration of heparin that should be used to heparinze
syringes prior to blood withdrawal?"
If you use heparinized collection tubes such as Microtainer, Starsedt,
you don't have to heparinize the syringes. Even adding heparin (20-25
units) to blank blood tubes/eppendorfs using a repeat pipettor is
typically more time efficient/easier than adding it to each syringe.
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