Dear All,Back to the Top
I would like to check the in vitro human and rat plasma hydrolysis
rates for a series of newly synthesised drugs.
But, before, I would like to check the plasma esterase performances.
In particular, their susceptibility to temperature and storage.
1) Should I centrifuge to obtain plasma at 4\0x221EC (3000g, 10 min) or room
temperature is OK as well?
2) Should I aliquot immediately the plasma and store at -70\0x221EC until use
or could I work for 4 hours keeping it at 4\0x221EC?
3) If I keep plasma at room temperature for a day, what happens to
Briefly, in order to keep the esterase activity, should I be extremely
careful with temperature or not?
Until then I worked with a liver esterase bought from Sigma and it was
easy to handle (-20\0x221EC storage; work at 4\0x221EC for the whole morning).
I am also looking for references on in vitro half-life of hydrolysis in
human plasma for a Drug Index molecule to check the correspondence.
Any suggestion would be sooo appriciated!
Universit\0x2021 degli Studi di Parma
FedericaBack to the Top
Answers to your questions,
1. room temperature is ok
2. It is ok to keep the plasma samples at 4 degrees for 4 hrs
3. Although it is not advisable to keep the samples at room temp all
day, from my personal experiene, may be 10% loss.
S. SATHEESH ANAND, Ph.D
RESEARCH TRIANGLE PARK, NC - 27709
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