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Now a days i am working on siRNA formulation and i am facing a problem
of quanfication of siRNA.UV absobance at 260nm is the best method to
detect siRNA but i have few other components that are also UV
detectable especially at 260nm.
I have few questions regarding siRNA
1.Is siRNA is stable in PBS(Phospate buffer saline)?
2.If its not stable in PBS then which buffer is suitable?
3.Is fluroscent labeled siRNA is stable in PBS and its quantification
4.If the quantification is by agaroelectrophorosis then what is the
percentage of agarose?
I would appreciate if someone is having experience in this.
Graduate Research Student
Department of Pharmaceutics
Wayne State University
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