Dear allBack to the Top
I am trying to equilibrate the mobile phase, ammonium acetate buffer 10
mM/acetonitrile (95:5 v:v) in the Hplc system but the zero over lamp
lights up and the zero adjustment is not affected. When I switch this
mobile phase to methanol: H2O 50:50, the zero adjustment is working.
Could you tell me what I have to do to solve this problem.
University Of Iowa
Dear Sayer,Back to the Top
Try using potassium phosphate or sodium phosphate buffer instead
of ammonium acetate
buffer in your mobile phase. I think this will work.
Dear SayerBack to the Top
Pl check the detector setting in your HPLC method. Normally, Detector
on/off is not related to mobile phase.
Sr. Research Associate
Zydus Research Centre, Ahmedabad
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