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Dear group members
My major part of work related to absorption and metabolism studies.
my drug shows good activity in invitro but it fails in invivo, the
major problem is solubility. In water it shows 300 ng/ml.
Sir present I am doing the rat insitu model for my permeability
study, I have used 5% organic as methanol in the perfusion buffer,
so far I have performed the experiment along with known markers, I
got good results for markers ,in case of my drug I am not able to
detect it, earlier I thought my drug was completely absorbed. And
even I have perform the adsorption to the tubing , syringe and photo
stability . the result shows drug was not having any adsorption
problem and it is stable in the light.
I can't increase the concentration more than 500 ng/ml( this
concentration I am perfusing in the rat intestine' jejunum" part),
and there is uneven distribution of the drug during the one hour
expt, so I am not sure about my results, it may be absorbed or the
same concentration was not allowed during the sample collection.
I have tried with different surfactants to increase the solubility,
that I can increase my concentration in my expt, here the results are
not fruit full.
Finally I performed one expt with 5 % organic as DMSO in my
perfusion buffer and here I found some good results and I can go up
to 2 ug/ml con in my perfusion buffer and even the drug distribution
is stable up to three hours, so I am happy with this results
Now my doubt -" can I use 5% organic as DMSO in my permeability
expt" and is it effects on P-Gyp" because i am doing the both expt's
plz clarify my doubts
Thanking you sir
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