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The following message was posted to: PharmPK
Dear all,
Kindly give the information about quantification
method for "felodipine" in human plasma for my
research purpose, i tried so many sites and i tried so
many methods but still not getting the good response.
in all the methods i am getting matrix effect, i am
tring the range from 0.020-800 ng/ml. if you have any
information about this please send.
regards
P.L.Mohan Kumar.
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1.extraction: 0.5 ml plasma + 0.1 ml NaOH 0.2M + 6 ml Et2O:Hexane
75/25, vortex, separation, evap.
2. chrom: 0.1% formic acid / acetonitrile
3. MS: on ion trap, 384>338 or 352, evaporation temp must not be too
high!!! (me: 280 C, at a flow of 1 ml/min)
4. on an ion trap you have linearity on range 0.02- 4 ng/ml, not 800
(!), maybe only on triple quad
regards
laurian
Vlase Laurian
MD, PhD, Pharm. Chem.
Teaching Assistant
Dept. of Pharmaceutical Technology and Biopharmaceutics
Faculty of Pharmacy
University of Medicine and Pharmacy "Iuliu Hatieganu"
13, Emil Isac
Cluj-Napoca, Cluj 400023, Romania
email:vlaselaur.-a-.yahoo.com
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