PharmPK Discussion - Fluoresence test for Hydrotrope

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On 21 Jun 2005 at 17:41:19, Amit Kulkarni (kulkara.at.email.uc.edu) sent the message


The following message was posted to: PharmPK

Hi,

I am looking at the solubilizing mechanisms of Hydrotropes
and Surfactants of Hydrophobic drugs and their effects on
Cell Membranes and Transporters.

I am trying to find information to characterize hydrotropes
from surfactants using fluoresence based or microscopy
assays.

Any help or literature references would be appreciated.

Amit Kulkarni
Graduate Student
Department of Pharmaceutical Sciences
University of Cincinnati
Phone (513) 558 4242

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On 22 Jun 2005 at 07:32:57, "Frank Bales" (frankbales.aaa.msn.com) sent the message


The following message was posted to: PharmPK

Dear Amit Kulkarni, regarding testing for Hydrotropes,

The simplest test for hydrotropes I have found is to observe the
crystalline "texture" obtained by re-crystallizing from water. Since
hydrotropes are a form of generalized liquid crystal, the resulting
"texture" is diagnostic.
An introduction to the literature is "Aggregation Behavior of
Hydrotropic Compounds in Aqueous Solution", Balasubramanian, D.
et.al. J. Phys. Chem. 1989, 93, 3865-3870.
If your task is to separate surfactants from hydrotropes in mixtures,
you should note that such mixtures can take the form of "mixed
micelles" that are distinct from the form of either material separately.
Hope this helps,

Frank
Frank Bales, Ph.D.
Email: frankbales.-at-.msn.com

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