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Does anyone have any tips to help "stabilise" a newly-formed
O-glucuronide. I have found that I lose ca. 75% of conjugate (back to
the corresponding aglycone) in ca. 16h at +4oC in incubation
supernatant from initial analysis. The incubation media was : 50mM
phosphate buffer + 3mM MgCl2 + 1mM EDTA + alamethicin (ca. 50ug/mg
microsomal protein) + 5mM UDPGA + 5mM saccarolactone + 1mM aglycone.
The product is not an acyl glucuronide.
I need to sort this out before scale-up.
Thanks in advance.
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