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The following message was posted to: PharmPK
What are the normal values of serum level of : GOT,GPT
in Balb/c mice (20grams in weight) ?
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I'm Setyo from Indonesia.
I've asked before about normal values of SGOT and SGPT of Balb/c
mice, and in archive index I knew that information can be found in
textbook of "Clinical biochemical and hematological references values
in normal experimental animals" by Mitruka, M and Rawnsley, HM. But I
couldn't find that book in my town's libraries. To get that book in
big city, it was very difficult problem because I just have limited
time and money, of course. I must finish my assignment soon. Could
you help me to find out normal values of SGOT and SGPT of Balb/c mice?
Thank you very much.
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The following message was posted to: PharmPK
Dear Setyo,
I do not posses reference values you asked for but I was wondering if
you do have Balb/c mice why
don't you determine reference interval yourself? If sample volume is
not a restriction draw and
analyze samples from animal population that you plan to include in
your pharmacological experiment
before the experiment to determine baseline SGOT and SGPT values. The
easiest way to calculate
reference values is to divide experimental values into classes and
calculate class frequencies.
Remove 2.5% tails from contingency tables obtained in described way
and you have reference interval
you need. The alternative is to calculate confidence intervals based
on assumption about normal or
some other distribution of experimental values. Possible problem that
could arise is related to too
small group of animals. If so you could analyze significance of
differences between SGOT/SGPT values
obtained before pharmacological experiment and during the experiment.
Problem with small number of
animals in this case could be cured to some extent by application of
t-test (one-way ANOVA) or
rerandomization test for dependant samples (such tests have higher
statistical power than their
independant samples counterparts). Of course, this proposal is not
equivalent to reference interval
approach, but it gives better insight in nephro- or hepatotoxicity of
analyzed drugs. Hope this
helps.
Zeljko debeljak, PhD student
Osijek Clinical Hospital
CROATIA
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Copyright 1995-2010 David W. A. Bourne (david@boomer.org)