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The following message was posted to: PharmPK
I have appreciated the several messages in the past day or so on the
use of HSA-LC or HSA-LC/MS for proten binding screening, and I posted
a message regarding our use of the column.
Has anyone used the columns for competitive experiments to examine
For example, as expected we see a long retention for warfarin
(separation of the isomers with retention of 9.4 and 14.8 minutes).
If we co-inject a compound that binds at the "warfarin site" on HSA,
shouldn't we see a retention time shift for warfarin (to an earlier
retention)? Are there sufficient binding sites on the HSA columns
that an appreciable amount (superclinical concentrations?) of
warfarin or another compound(s) are required to observe the effect?
Looking forward to any replies.
Associate Scientist, Drug Metabolism and Pharmacokinetics
The Woodlands, TX 77381
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