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Dear All,
I am planning to determine rocuronium concentration in plasma using
an LC-MS method. My question is whether treating plasma with sodium
dihydrogen phosphate is crucial for drug stability upon freezing, and
what effect this step may have on the sensitivity of the MS detection.
Regards,
Khalid Alkharfy
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The following message was posted to: PharmPK
Dear Dr. Alkharfy,
Our lab has extensive experience in the determination of rocuronium
concentration in plasma. E.g. see our paper:
Kleef UW, Proost JH, Roggeveld J, Wierda JMKH. Determination of
rocuronium
and its putative metabolites in body fluids and tissue homogenates. J
Chromatogr 1993;621:65-76.
In this paper we described that plasma samples with rocuronium are
stable
at -20 C for at least 9 months. Based on these results we recommend
not to
acidify plasma samples of rocuronium, thus avoiding possible pipetting
errors and inaccuracies.
Best regards,
Hans Proost
Johannes H. Proost
Dept. of Pharmacokinetics and Drug Delivery
University Centre for Pharmacy
Antonius Deusinglaan 1
9713 AV Groningen, The Netherlands
tel. 31-50 363 3292
fax 31-50 363 3247
Email: j.h.proost.at.rug.nl
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