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I am studying TPMT activity in erythrocyte lysates by measuring the
formation of 6MMP from 6MP, using HPLC . I had some problems with the
stability of S-adenosylmethionine. I want to know if anyone have some
experience with the stability (or storing conditions) of this drug
(stored at -20C), or the stability of SAM solution (stored at -70C).
Thank you,
Dr Guillermo Bramuglia
Profesor Adjunto
Catedra de Farmacologia,
Facultad de Farmacia y Bioquimica,
Universidad de Buenos Aires
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The following message was posted to: PharmPK
Hola.
I think that you have to add Dithiotreitol (DTT) to the SAM stock
solution (1 mM according to Indjova et al, Ther Drug Monitoring 25:637.
2003) to prevent oxydation of the thiol group.
Suerte!.
Facundo Garcia Bournissen.
facugb3.aaa.hotmail.com
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