Back to the Top
The following message was posted to: PharmPK
Hi,
a few years back there was a scare that vacutainer use to collect blood
for
plasma preparation was resulting in reduced drug level measurements due
to
adsorption onto the matrix. there was at least one paper published. Is
this
still a concern ?
Thanks
Dave
Back to the Top
The following message was posted to: PharmPK
There was an issue where the pasticizer, Tris Butoxy Ethyl Phosphate
(TBEP)
was leached from the stoppers into the blood sample and subsequently
displaced anionic compounds like tricyclic antidepressants from plasma,
that
were subsequently sequestered in the RBCs. On centrifuging and
separation,
some of the compound was discarded in the erythrocytes. As a matter of
good
practice, you should perform a whole blood lysis vs plasma comparison to
exclude RBC partitioning whether from displacement by extraneous
factors;
i.e. pasticizer, anticoagulants (heparin, EDTA) or inherent properties
of
the compound. TBEP is a rather ubiquitous contaminant from many
sources, IV
bags, syringe plungers, etc.
Cordially,
Will
PharmPK Discussion List Archive Index page
Copyright 1995-2010 David W. A. Bourne (david@boomer.org)