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Hello
I am not sure if this where to post this. I am undertaking Km
substrate determinations At different protein concentrations and
substrate concentrations at set time. Unfortunately a number of my
substrates show inhibitory activity. I have been told that I can use
this equation Y=Vma*X/(Km+X(1+X/Ki)) to determine the Km and Ki with
the above plot with Prism. I have not come across this before. I
would have thought you would have to use a Lineweaver Burk Plot.
Chris Wells
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