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Hi everybody.
I want to know if anyone can suggest a method or a reference for
quantifying perindopril and its metabolite perindoprilate in human
plasma. Our Cmax is for perindopril 2 ng/ml to 35ng/ml:for
perindoprilat 0.2ng/ml to3.5ng/ml and we would like to
achieve a LOQ of at least 0.1 ng/ml with good recovery. We are
using an SPE extraction
method to isolate the drug from plasma but are facing problems with
recovery of the analyte in the C18 sorbent bed.
What other sorbents can you suggest for this&any extraction method
Thank you
rajanikanth
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The following message was posted to: PharmPK
Rajanikanth,
I would use Oasis HLB instead of a C18 sorbent. Perindopril and its
metabolite are very hydrophilic molecules so you won't have a good
retention
in C18 sorbents. You probably faced problems with LC profile. I hope
this
help you.
Rafael E. Barrientos Astigarraga
MAGABI Pesq. Clin. Farm. Ltda.
Tel. (+55-11)5521 0232
rafael.barrientos.aaa.magabi.com.br
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