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I am trying to prepare Rat Intestinal Membrane Preparations so that I
can use the membrane fraction for radioligand binding assay. The
protein is a large transporter and is quite susceptible to
proteolysis. I have to have the intact protein present in the
membrane for the expt to work. I am very new to this thing, so I was
wondering whether anyone can give me some pointers or advice of how
to make good membrane preps and how to assay for how good the prep is.
I will truly appreciate your detailed advice (specially what kind of
buffer to use, how to lyse the cells etc) on this.
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