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Hi all,
Imipenem is an iv beta lactam antibiotic co-prescribed with
cilastatin ( a dehydropeptidase inhibitor).
Plasma samples as well as stock solution in the solvent of imipenem
is not stable.
Could you please suggest me how to stabilize the compound in plasma/
blood? Does it show concentration dependent stability particularly
when tried to stabilize in MOPS buffer?
Bhagwat Prasad
NIPER
INDIA
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Dear Mr. Bhagwat,
Firstly i am not able understand the line written by you as "Plasma
samples as well as stock solution in the solvent of imipenem is not
stable". What solvent of imipenem stands for?
Regards
Vineet Singh
Vineet Singh
Senior Research Fellow
Department of pharmacy
S.G.S.I.T.S., Indore
[What is the solvent? - db]
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The following message was posted to: PharmPK
Hi Bhagwat,
I made experiments on imipenem by microdialysis, its degradation to
the temperature (10% degradation at 25 degrees C after 3.5h) and the
light should be considered: by keeping it in an ice bath, using brown
tubes and syringes, preparing solutions (in 0.9% NaCl and Ringer)
immediatly prior to the administration, centrifuging (at 3,000 rpm
for 10 min at 4*C) collected blood samples (in heparinized tubes) and
freezing (-20*C) supernatants immediately after withdrawing and
diluting microdialysis dialysates with a stabilizer (0.5 M HEPES
buffer, pH 6.8; ethylene glycol; HPLC-grade water [1:0.5:0.5; vol/vol/
vol]) immediately after collection.
Hope it helps.
Regards.
elodie PLAN
Stagiaire, GMPK
sanofi-aventis
Alfortville, FRANCE
01-58-93-86-88
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Dear sir,
I mean stability of imipenem in solution form (irrespective of solvent).
Regds
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