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I am in the process of developing protein binding assay by
equilibrium dialysis method. I faced the problem of volume shift from
buffer compartment to plamsa compartment.
I went through the archive of pharmpk and found that this is well
known old problem, and if any of you have got the pdf format of these
following article, could be send me the same. It would be of great help.
This is a well known old problem with this technique and is one of
thedrawback of this method. Go through these papers to solve your
1: Lapicque F, Netter P, Monot C, Bannwarth B, Maknassi MS, Royer
RJ. [Determination of protein binding of drugs using equilibrium
dialysis.Influence of volume displacement caused by osmotic pressure]
J Pharmacol. 1986 Jul-Sep;17(3):295-300.French.
2: Curry SH, Hu OY. Evaluation of equilibrium dialysis volume
shifts: a comment.J Pharmacokinet Biopharm. 1984 Aug;12(4):463-5.
3: Tozer TN, Gambertoglio JG, Furst DE, Avery DS, Holford NH.
Volume shifts and protein binding estimates using
equilibriumdialysis:application to prednisolone binding in humans.J
Pharm Sci. 1983 Dec;72(12):1442-6.
4: Huang JD. Errors in estimating the unbound fraction of drugs due
to the volumeshift inequilibrium dialysis.J Pharm Sci. 1983 Nov;72
(11):1368-9. No abstract available.
5: Lima JJ, MacKichan JJ, Libertin N, Sabino J. Influence of volume
shifts on drug binding during equilibriumdialysis:correction and
attenuation.J Pharmacokinet Biopharm. 1983 Oct;11(5):483-98.
Thanks for your help.
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