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I was curious if anyone had any information they may be willing to
share with regard to using zinc sulfate to lyse blood samples prior
to extraction and any effect this may have on the resulting data if
one were looking to examine API as well as metabolites?
Thanks for your time and consideration,
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The following message was posted to: PharmPK
Regarding Zinc Sulfate to Lyse Blood: You take me back to about 1970
when I assayed isoniazid in blood. Please refer to the following two
publications: (1) Boxenbaum, H.G. and S. Riegelman. Determination of
isoniazid and metabolites in biological fluids. J. Pharm. Sci. 63:
1191 1197 (1974); and (2) Scott EM, Wright RC, J Lab Clin Invest
70:355-?, 1967. What is nice about this method is you get a very
clean, virtually neutral solution. You can modify this procedure
according to your needs. Here goes:
One-half mL. heparinzed blood is placed in a culture tube containing
15.0 mL distilled water. This will lyse the blood cells after 45 or
more seconds. Then add 1.00 mL zinc sulfate solution and shake by
hand for 5 seconds. Then add 1.00 barium hydroxide solution and
shake vigorously for about 5-10 seconds. Immediately filter through
Whatman #1 filter paper. You get a clear, colorless filtrate, have a
pH near neutral, with littler buffer capacity.
The zinc sulfate solution is approximately 5% zinc sulfate-7-water W/
V, and the barium hydroxide solution is approximately 0.30 N.
Good luck. Write me if you have questions. Best wishes. Harold
Harold Boxenbaum, Ph.D.
14621 Settlers Landing Way
North Potomac, MD 20878-4305
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