PharmPK Discussion - FD4 for intestinal permeability assessment

• On 5 Jan 2007 at 10:46:00, "David Foster" (drfoster.-at-.iupui.edu) sent the message
  

  
  The following message was posted to: PharmPK
  
  Hello. I would like to use FD4 (fluoresceinisothiocyanate-dextran) in
  a rat
  single perfusion model, however I do not have a fluorescence
  spectrofluorometer in my lab.  Does anyone know if FD4 is stable when
  frozen
  and stored (i.e., I could store the samples for batch
  analysis)....thanks...Dave
  
  David R. Foster, Pharm.D.
  Assistant Professor of Pharmacy Practice
  Purdue University, School of Pharmacy and Pharmaceutical Sciences
  Wishard Health Services, W7555 Myers Building
  1001 W. Tenth Street
  Indianapolis, IN 46202-2879
  (317) 613-2315x309, fax (317) 613-2316
  
  

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• On 5 Jan 2007 at 10:54:55, "Ed O'Connor" (Ed.OConnor.aaa.tandemlabs.com) sent the message
  

  
  The following message was posted to: PharmPK
  
  I used FITC inulin in rat plasma.  We could freeze up to 3 cycles -80.
  Just need to demo FT and long term stability.
  
  Ed O'Connor, PhD
  Technical Director, Immunoanalytical
  Tandem Laboratories
  115 Silvia Street
  West Trenton, New Jersey
  609-228-0243
  
  

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• On 5 Jan 2007 at 13:38:51, "Murad Melhem" (melhemmr.-at-.gmail.com) sent the message
  

  
  The following message was posted to: PharmPK
  
  Dear Dr. Foster,
  
  In the 11th annual FDA Science Forum (2005), a poster was presented by
  Hunt , Faustino and Volpe on the stability of in vitro stock
  solutions. Various drug and reagent solutions were tested.
  
  The abstract reads that even though, FITC-dextran (FD4) stock was
  stored at at -80=B0C, the content decreased 7% after 2 weeks. This
  should be accounted for even though it does not sound very
  significant.
  
  It should be noted though that stock solutions were tested by that
  group. Diluted working solutions are generally expected to be less
  stable.
  
  I think it can [it can - db] be found through a listing by authors in =20=
  
  the following
  website; but I am copying [listing - db] the abstract below just in =20
  case:
  
  http://www.accessdata.fda.gov/scripts/oc/scienceforum/sf2005/Search/=20
  coauthorindex.html
  
  I hope this is of help.
  
  Murad Melhem, Ph.D.
  Cognigen Corporation
  Buffalo, NY
  
  --
  Board Number: H-09
  Title: Stability screening of in vitro stock drug solutions
  Authors: R. L. Hunt , P. J. Faustino , D. A. Volpe , DPQR/CDER/FDA, =20
  Silver Spring, MD
  
  

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