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Hi,
I would like to calculate in-vitro intrisnic clearance using liver
microsomes data. I have calculated T(1/2) from K. The liver
microsomes protein concentration was 1.0 mg/mL.
Is this right? CLint (in-vitro) = 0.693/(T1/2 in hr)*(mg protein).
What will be the units?
Thank you
Sue
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The following message was posted to: PharmPK
Hi Sue,
The equation people used earlier was:
CLint = (V * 0.693)/(T1/2 * mg protein)
Where V is incubation volume.
T1/2 is In-vitro half life.
Provided 45 mg microsome/gram of tissue and 20 gm of tissue / kg body
weight.
The final units for CLint will be "L/hr/kg" by deriving from above
values.
However there are other equations found if you search in google.
Here are the links that were discussed in this PharmPK forum way back
in 2000.
www.boomer.org/pkin/PK00/PK2000107.html
www.aapspharmaceutica.com/meetings/files/36/Gan.revised.092804.pdf
Hope this helps to some extent.
Thanks
Sripal
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