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Dear Pharm PK members,
I am working on fluorescent-based HPLC method development of
irinotecan. The excitation wavelength is set at 370 nm and emission
scans are recorded in different acidified solvents (0.1%
trifluoroacetic acid in acetonitrile, 0.1% glacial acetic acid in
acetonitrile and 7% perchloric acid in methanol) for wavelength
selection. What I see is that the RFU value decreases with time in
all solvents. Why is this happening and how can this decrease be
avoided. Also, I am not able to get linearity in response at
different irinotecan concentrations at a selected wavelength (Ex 370
Em 420) in these acidified solvents. Kindly advice. I haven't worked
much on fluorescence detector.
Thanks and Regards
Tripta
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