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The following message was posted to: PharmPK
Dear Group,
If you have any experience with K3EDTA before, could you please let
me know the concentration and amount of K3EDTA that is usually used
as anticoagulant, and its source.
thanks in advance
Ying
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I have previous experience with EDTA disodium salt. In our studies
the the concentration of EDTA as an anticoagulant (also tested as a
sample stabilizer) was 4mM (1.5 mg/mL): Samtani MN, Jusko WJ.
Stability of dexamethasone sodium phosphate in rat plasma. Int J
Pharm. 2005 Sep 14;301(1-2):262-6.
You can find additional information on the sigma webpage: http://
www.sigmaaldrich.com/sigma/product%20information%20sheet/e6511pis.pdf
Sigma sells this product as a 2% EDTA solution. 1-2 drops (75-150 uL)
per ml of whole blood can be used as an anticoagulant. The 2% EDTA
solution webpage is: http://www.sigmaaldrich.com/catalog/search/
ProductDetail/SIGMA/2854
Good luck...Mahesh
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Dear Ying,
Typically 2% of K3EDTA solution is needed. Then same is used as 10%
of require blood volume.
Samiulla
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Dear Ying,
Generally K3 EDTA will be used at 15 % concentration and at this
concentration only 0.1 to 0.12 % of the volume is required.
This is the concentration one could find in Vacutainers used to
collect the blood samples to get the plasma sample.
Hope this helps
RajaReddy
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Dear Ying
It is 1 to 1.2 % of the volume required not 0.1 to 0.12 %.
Thanks
Raj
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