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Hello,
I am currently developing a method for detecting a compound in tissue
samples. I do not have any experience with solid samples, how do I
"spike" my standards into the tissue (liver or kidney) to develope an
extraction method? any suggestions?
Thanks,
Steve
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The following message was posted to: PharmPK
Dear Stephen ,
It is advisable to add your standards as early as possible during sample
clean up (You never know). That means, e.g. chop your tissue, put it in
your extraction/homogenisation medium, add your standards and start with
your procedure (Ultraturrax, Potter or glass beads etc.). Please
think of
the fact that you will not see if your compound is properly extracted
out
of the cells. You have to apply a homogenisation/extraction that is
suitable for this purpose. Hopes this helps a little bit.
Kind regards
Thomas
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The following message was posted to: PharmPK
Dear Steve
For tissue samples you can first weigh the tissues and you add the
diluent which your using (generally buffer or normal saline). But as
there is variation in the weight in each time for every tissue so you
can fix the ratio of weight of tissue : diluent.
Homogenised this mixture. After that the homogenised mixture you can
use for spiking like plasma spiking what your following.
PRASHANT
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