PharmPK Discussion - P-glycoprotein western blotting

• On 16 Apr 2007 at 16:57:20, "Noha Salama" (nohasalama.aaa.gmail.com) sent the message
  

  
  Hi
  Can anyone suggest a ratio for using RIPA buffer to lyse Rat tissues
  (liver, kidney, duodenum, etc..) to extract P-glycoprotein for
  Western blotting detection? Any suggestions of any other lysis buffer?
  if we have tissue homogenate in trizol, can we use these samples as
  such to be mixed with the sample laemelli buffer for Western blotting?
  Any advice is greatly appreciated
  thanks
  Noha
  
  Noha Nabil Salama, Ph.D.
  Clinical Research Fellow
  The University Of Michigan-College of Pharmacy
  Department of Clinical Sciences
  
  

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