PharmPK Discussion - Predicting optimum buffer concentration

• On 11 Jan 2007 at 09:23:29, "sankaratata" (sankaratata.aaa.indiatimes.com) sent the message
  

  
  The following message was posted to: PharmPK
  
  Dear all,
  
  I would like to know any theories /  methods that be used to predict
  the optimum concentration of a buffer for mobile phase preparation in
  Chromatographic seperation.
  
  Thank you all in advance
  
  

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• On 11 Jan 2007 at 08:52:56, "Michele Sanders" (mes.aaa.sparhawklabs.com) sent the message
  

  
  The following message was posted to: PharmPK
  
  RE: PharmPK Predicting optimum buffer concentration
  
  Without knowing your analyte and instrumentation (e.g. UV-HPLC vs. LC/
  MS) I
  hesitate giving broad-spectrum advice.  But, below are some places I
  go when
  I need to refresh on "buffer-ology."
  
  http://www.chromforum.com/ is a chromatography discussion board.
  Experts from the
  chromatography field, end-users, and vendors all contribute to the
  discussions on the site.
  
  http://www.zirchrom.com/pass.asp has a buffer wizard.
  
  http://www.mac-mod.com/pdf/TR-BufferedMP.pdf is a Mac-Mod report on
  preparing mobile phase buffers for reversed phase chromatography.
  
  Hope this helps.
  
  

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• On 11 Jan 2007 at 08:00:13, "Ed O'Connor" (Ed.OConnor.aaa.tandemlabs.com) sent the message
  

  
  The following message was posted to: PharmPK
  
  Go to http://www.acdlabs.com/ they have an online suite subscription
  service to model chromatography with buffer manipulation
  
  Ed O'Connor, PhD
  Technical Director, Immunoanalytical
  Tandem Laboratories
  115 Silvia Street
  West Trenton, New Jersey
  609-228-0243
  
  

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