PharmPK Discussion - Thiols analysis

• On 24 Jul 2007 at 19:14:11, "Katya Tsaioun" (katya.-at-.apredica.com) sent the message
  

  
  The following message was posted to: PharmPK
  
  Does anyone have experience getting very small thiols to ionize in
  ESI source?
  APCI? Derivatization?
  
  Any ideas would be appreciated.
  
  Katya Tsaioun, Ph.D.
  President
  APREDICA
  313 Pleasant Street, 1st Floor,
  Watertown, MA 02472
  
  

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• On 31 Jul 2007 at 15:02:33, "Akshanth Polepally" (akshanth.-at-.gmail.com) sent the message
  

  
  Dear Dr. Katya Tsaioun,
  
  Following are the articles, which may be helpful for you.
  
  1. Biomed.Chromatography. 17: 285-291 (2003)
       Bioanalytical considerations for compounds containing free
  sulfhydryl groups.
  
  2. Jemal M and Hawthrone D. Journal of chromatography B 1997a: 693: 107
  
  3. Jemal M and Hawthrone D. Journal of chromatography B 1997b: 698: 123
  
  4.Jemal et al. Anal Chem 2001; 73(22): 5450
  
  with best regards
  Akshanth
  DMPK
  Dr. Reddys labs
  
  

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• On 31 Jul 2007 at 19:55:03, "RAJAN K S" (komburajan.-a-.gmail.com) sent the message
  

  
  Dear Dr. Tsaioun,
  If you are talking about cysteine kind of molecules, you can easily
  derivatize it using 10 mM iodoacetic acid in 10 mM ammonium
  bicarbonate at pH10. If you suspect autooxidation, you may use
  dithiotheritol in the same buffer. For ESI analysis, you may use 0.1%
  formic acid in water and in acetonitrile with a suitable column.
  Hope this is helpful,
  Regards,
  Rajan Kombu
  
  Research Associate
  Case Western Reserve University,
  2109 Adelbert Road, Cleveland, OH-44120
  
  

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