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I am working on some compound for which I require charcoal stripped
plasma. Can anybody suggest me how to get charcoal stripped plasma.
Can it be obtained by doing some procedure with the normal plasma.
Another thing is it available commercially any where in India.
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Here is the procedure: "Plasma based standards and quality control
standards were prepared from rat plasma, which had been stripped of
endogenous corticosterone using Norit-A pharmaceutical-grade neutral
decolorizing carbon (Amend Drug and Chemical Co., Irvington, NJ).
Charcoal was added to rat plasma at the concentration of 4 g/100 mL.
The suspension was stirred at room temperature for 8 hr and
centrifuged overnight at 35300 x g in a Beckman J2-HS centrifuge at
4*C. Plasma was filtered using non-sterile Acrodisc filters (Gelman
Sciences, Ann Arbor, MI) attached to a 20 cc syringe in the following
sequence: 5 um, 1.2 um, 0.45 um."
Ref. Samtani MN, Jusko WJ. Quantification of dexamethasone and
corticosterone in rat biofluids and fetal tissue using highly
sensitive analytical methods: assay validation and application to a
pharmacokinetic study. Biomed Chromatogr. 2007 Jun;21(6):585-97.
I think Lampire Biological Laboratories (Pipersville, PA) is one of
the vendors of charcoal stripped plasma but it is much cheaper to buy
the plasma and strip it of endogenous (interfering) molecules using
pharmaceutical-grade neutral decolorizing carbon as described above.
I hope this is helpful...Mahesh
Mahesh N. Samtani, Ph.D.
Clinical Pharmacology, Advanced PK-PD M&S
Johnson & Johnson Pharmaceutical R&D
920 Route 202, PRD 2723, Raritan, NJ 08869
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